Analysis of RNA-seq samples

Sequenced RNA-seq sampled are analyzed for disease identification and RNA-edit checking. Many stacked pipelines are used for this analysis. GATK, the Genome Analysis Toolkit from Broad Institute has a elaborated pipeline for sequence analysis. SPRINT, RNA edit identifying tool has integrated pipeline for sequence analysis. Normally, sequences are first mapped for aligning using several aligning tools, specially found one is BWA (Burrows-Wheeler Aligner). There are several other multi optioned analysis tools like, bcftools, vcftols, bedtools. In found RNA-edit there is also DNA mutations, and these can be filtered using dbSNP.

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